In situ product recovery as a powerful tool to improve the fermentative production of muconic acid in Saccharomyces cerevisiae

Muconic acid has gained interest as a bio-based platform chemical to produce value-added compounds including adipic and terephthalic acid. In this study, fermentation strategy based on in situ product recovery was established boost process performance counteract inhibition. An optimized reactive extraction mixture for muconic developed consisting of 12.5% (v/v) CYTOP 503 canola oil showing high capacity well biocompatibility. By integrating the into at shake flask level, growth titer could be increased by 44% 18%, respectively. The successfully transferred fed-batch 10 L bioreactor with final 4.33 g L−1 maximum productivity 0.053 L−1, demonstrating potential enhance challenging processes industrial biochemicals.